Biotechnology and Its Applications PYQs

The human protein named α-1-antitrypsin, obtained from transgenic animals, is used for the treatment of ______.

Match List I with List II: Choose the correct answer from the options given below:

The toxin proteins isolated from Bacillus thuringiensis, coded by which genes control cotton bollworms and corn borer, respectively?

Given below are two statements : Statement I : Transfer RNAs and ribosomal RNA do not interact with mRNA. Statement II : RNA interference (RNAi) takes place in all eukaryotic organisms as a method of cellular defence. In the light of the above statements, choose the most appropriate answer from the options given below:

Which of the following genetically engineered organisms was used by Eli Lilly to prepare human insulin?

Identify the part of a bio-reactor which is used as a foam breaker from the given figure.

Silencing of specific mRNA is possible via RNAi because of -

Why can’t insulin be given orally to diabetic patients?

Given below are two statements: Statement I: Low temperature preserves the enzyme in a temporarily inactive state whereas high temperature destroys enzymatic activity because proteins are denatured by heat. Statement II: When the inhibitor closely resembles the substrate in its molecular structure and inhibits the activity of the enzyme, it is known as competitive inhibitor. In the light of the above statements, choose the correct answer from the options given below:

Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment?

If a geneticist uses the blind approach for sequencing the whole genome of an organism, followed by assignment of function to different segments, the methodology adopted by him is called:

Transposons can be used during which one of the following?

In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because:

Statements related to human insulin are given below. Which statement(s) is/are correct about genetically engineered insulin? (a) Pro-hormone insulin contains extra stretch of C-peptide (b) A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them (c) Insulin used for treating diabetes was extracted from cattle and pigs (d) Pro-hormone insulin needs to be processed for converting into a mature and functional hormone (e) Some patients develop allergic reactions to foreign insulin Choose the most appropriate answer from the options given below:

Match List-I with List-II

When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as:

Identify the incorrect pair:

For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection? (1) Hybridization Technique (2) Western Blotting Technique (3) Southern Blotting Technique (4) ELISA Technique

With regard to insulin choose correct options. (a) C-peptide is not present in mature insulin. (b) The insulin produced by rDNA technology has C-peptide. (c) The pro-insulin has C-peptide. (d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges. Choose the correct answer.

In gel electrophoresis, separated DNA fragments can be visualized with the help of

Match the organism with its use in biotechnology.

Which of the following statements is not correct?

Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to:

Match the following columns and select the correct option.

Exploration of molecular, genetic and species level diversity for novel products of economic importance is known as:

In mung bean, resistance to yellow mosaic virus and powdery mildew were brought about by:

Exploitation of bioresources of a nation by multinational companies without authorization from the concerned country is referred to as

Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called:

In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is:

A ‘new’ variety of rice was patented by a foreign company, though such varieties have been present in India for a long time. This is related to:

Which kind of therapy was given in 1990 to a four-year-old girl with adenosine deaminase (ADA) deficiency?

Golden rice is a genetically modified crop plant where the incorporated gene is meant for biosynthesis of:

The introduction of T-DNA into plants involves:

The first human hormone produced by recombinant DNA technology is:

Commonly used vectors for human genome sequencing are:

To obtain virus-free healthy plants from a diseased one by tissue culture technique, which part/parts of the diseased plant will be taken?

Which of the following Bt crops is being grown in India by farmers?

Tobacco plants resistance to a nematode have been developed by the introduction of DNA that produced (in the host cells):

The first clinical gene therapy was given for treating:

Silencing of mRNA has been used in producing transgenic plants resistant to:

Which one of the following is now being commercially produced by biotechnological procedures?

Transgenic plants are the ones:

What is true about Bt toxin?

The genetic defect-adenosine deaminase (ADA) deficiency may be cured permanently by:

A transgenic food crop which may help in solving the problem of night blindness in developing countries is

Which one of the following is being tried in India as a biofuel substitute for fossil fuels?

Cry I endotoxins obtained from Bacillus thuringiensis are effective against

Modern detergents contain enzyme preparations of

Main objective of production/use of herbicide resistant GM crops is to

Human insulin is being commercially produced from a transgenic species of

What is antisense technology?

Golden rice is a promising transgenic crop. When released for cultivation, it will help in:

In order to obtain virus-free plants through tissue culture the best method is:

Which of the following is generally used for induced mutagenesis in crop plants?

Bacillus thuringiensis (Bt) strains have been used for designing novel:

Golden rice is a transgenic crop of the future with the following improved trait: