Biotechnology: Principles and Processes PYQs

Match List I with List II: Choose the correct answer from the options given below:

Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction:

Arrange the following steps of somatic hybridisation in a correct sequence. A. Digestion of cell walls. B. Isolation of naked protoplasts. C. Fusion of protoplasts to get hybrid protoplast. D. Isolation of single cells from two different varieties of plants. E. Growing of hybrid protoplast to form a new plant. Choose the correct answer from the options given below:

Which of the following statements are not true regarding restriction endonucleases? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the growth of bacteriophages in E. coli. C. They cut the DNA only at the centre of the palindromic sites. D. They remove nucleotides only from the ends of DNA fragments. E. They recognise specific palindromic base-pair sequences.

Which of the following statements are correct with respect to DNA separation, isolation and visualization? A. The cutting of DNA is done by molecular scissors. B. The DNA fragments separate according to their size in an agarose gel, upon electrophoresis. C. The separated DNA fragments can be seen without staining when exposed to UV light. D. The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light. Choose the correct answer from the options given below :

Insertion of a foreign DNA at BamHI site in an E. coli cloning vector pBR322 results in the loss of antibiotic resistance towards:

Polymerase chain reaction (PCR) amplifies DNA following the equation.

In the above represented plasmid an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes B. DNA ligase C. DNA mutase D. DNA recombinase E. DNA polymerase Choose the correct answer from the options given below :

The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate. Given below are two statements about this method: Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies. Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies. In the light of the above statements, choose the most appropriate answer from the options given below:

What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism? A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism. B. It may get integrated into the genome of the recipient. C. It may multiply and be inherited along with the host DNA. D. The alien piece of DNA is not an integral part of chromosome. E. It shows ability to replicate. Choose the correct answer from the options given below:

The capacity to generate a whole plant from any cell of the plant is called:

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

Which of the following are fused in somatic hybridization involving two varieties of plants?

The “Ti plasmid” of Agrobacterium tumefaciens stands for

Match List I with List II : Choose the correct answer from the options given below :

Which of the following statements is incorrect?

The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes:

Expressed Sequence Tags (ESTs) refers to

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

In gene gun method used to introduce alien DNA into host cells, microparticles of ________ metal are used.

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence. A. Insertion of recombinant DNA into the host cell. B. Cutting of DNA at specific location by restriction enzyme. C. Isolation of desired DNA fragment. D. Amplification of gene of interest using PCR. Choose the correct answer from the options given below :

Match List I with List II. Choose the correct answer from the options given below:

Which of the following is not a cloning vector?

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Polymerase chain reaction is used in DNA amplification. Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation. In the light of the above statements, choose the correct answer from the options given below:

Which one of the following statement is not true regarding gel electrophoresis technique?

In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?

Given below are two statements: Statement I: Restriction endonucleases recognise specific sequences to cut DNA known as palindromic nucleotide sequences. Statement II: Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site. In the light of the above statements, choose the most appropriate answer from the options given below:

Which of the following is not a desirable feature of a cloning vector?

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

Plasmid pBR322 has PstI restriction enzyme site within gene amp^r that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactosidase production and the recombinant plasmid is inserted in an E.coli strain:

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:

Choose the correct pair from the following.

The specific palindromic sequence which is recognized by EcoRI is

Identify the wrong statement with regard to Restriction Enzymes.

The sequence that controls the copy number of the linked DNA in the vector, is termed

A selectable marker is used to:

Given below are four statements pertaining to separation of DNA fragments using Gel electrophoresis. Identify the incorrect statements. (a) DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal. (b) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA. (c) Smaller the size of DNA fragment, larger is the distance it travels through it. (d) Pure DNA can be visualized directly by exposing to UV radiation. Choose correct answer from the options given below:

An enzyme catalysing the removal of nucleotides from ends of DNA is:

Match the following enzymes with their functions: Select the correct option from the following:

The two antibiotic resistance genes on vector pBR322 are for

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

The correct order of steps in Polymerase Chain Reaction (PCR) is:

The process of separation and purification of expressed protein before marketing is called

DNA fragments are

A gene whose expresson helps to identify transformed cell is known as

The DNA fragments separated on an agarose gel can be visualised after staining with

Stirred-tank bioreactors have been designed for

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

Which of the following is not a component of downstream processing?

Which of the following restriction enzymes produces blunt ends?

The DNA molecules to which the gene of interest is integrated for cloning is called:

The cutting of DNA at specific locations became possible with the discovery of:

An analysis of chromosomal DNA using the Southern hybridization technique does not use:

In vitro clonal propagation in plants is characterized by:

Which vector can clone only a small fragment of DNA?

DNA fragments generated by restriction endonucleases in a chemical reaction can be separated by

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

In genetic engineering, the antibiotics are used:

The figure below shows three steps (A, B, C) of polymerase chain reaction (PCR). Select the option giving correct identification together with what it represents?

Biolistics (gene-gun) is useful for:

Which one of the following techniques made it possible to genetically engineer living organisms?

Read the following four statements (A-D) about certain mistakes in two of them: (A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched. (B) Restriction enzymes are used in isolation of DNA from other macro-molecules. (C) Downstream processing is one of the steps of R-DNA technology. (D) Disarmed pathogen vectors are also used in transfer of R-DNA into the host. Which are the two statements having mistakes?

Which of the following are used in gene cloning?

In genetic engineering, a DNA segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as vector/vectors. (A) a bacterium (B) plasmid (C) plasmodium (D) bacteriophage

Somaclones are obtained by:

Which of the following plant species would you select for the production of bioethanol?

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

Gel electrophoresis is used for

The linking of antibiotic resistance gene with the plasmid vector became possible with

Microbes found to be very useful in genetic engineering are:

Restriction endonuclease:

Production of a human protein in bacteria by genetic engineering is possible because:

Which one of the following makes use of RNA as a template to synthesize DNA?